Search results for " sor"
showing 10 items of 408 documents
The Chemo- and Stereoselective Formation of Pallado- and Platinocryptophanes
2019
International audience
Environment and Space Rule, but Time also Matters for the Organization of Tropical Pond Metacommunities
2020
Metacommunities are dynamic systems, but the influence of time independently of environmental change in their configuration has been rarely considered. Temporary ponds are excellent ecosystem models, as they have well-defined boundaries in time and space; their communities are relatively isolated through a landscape matrix, and the progress of time leads to major changes through ecological succession and in habitat suitability related to hydroperiod dynamics. Therefore, strong temporal effects are expected to influence their metacommunity structure. We surveyed 30 temporary ponds along the dry tropical region of western Costa Rica and Nicaragua at three different moments of their hydroperio…
Sorting Motifs Involved in the Trafficking and Localization of the PIN1 Auxin Efflux Carrier
2016
In contrast with the wealth of recent reports about the function of μ-adaptins and clathrin adaptor protein (AP) complexes, there is very little information about the motifs that determine the sorting of membrane proteins within clathrin-coated vesicles in plants. Here, we investigated putative sorting signals in the large cytosolic loop of the Arabidopsis (Arabidopsis thaliana) PIN-FORMED1 (PIN1) auxin transporter, which are involved in binding μ-adaptins and thus in PIN1 trafficking and localization. We found that Phe-165 and Tyr-280, Tyr-328, and Tyr-394 are involved in the binding of different μ-adaptins in vitro. However, only Phe-165, which binds μA(μ2)- and μD(μ3)-adaptin, was found …
Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide
2016
Efficient production and secretion of recombinant proteins in mammalian cell lines relies in a combination of genetic, metabolic and culture strategy factors. The present work assesses the influence of two key genetic components of expression vectors (promoter and signal peptide) on protein production and secretion effciency in HEK293 cells expressing eGFP as a reporter protein. Firstly, the strength of 3 different promoters was evaluated using transient expression methods. Flow cytometry analysis revealed that the highest level of intracellular protein expression was found when eGFP was under the control of CMV promoter, being 3-times higher in comparison to the rest of the promoters teste…
The role of watercourse versus overland dispersal and niche effects on ostracod distribution in Mediterranean streams (eastern Iberian Peninsula)
2016
Abstract The processes behind the heterogeneous distribution of species involve a combination of environmental and spatial effects. In the spatial context, stream networks constitute appropriate systems to compare the relative importance of two dispersal modes in aquatic organisms: overland and watercourse dispersal. In the present study, we analyzed the distribution of ostracod species in a river network in the eastern Iberian Peninsula, with variation partitioning between environmental and spatial factors, using Moran and Asymmetric Eigenvector Maps (MEMs, AEMs) as spatial variables. Our aims were to determine the relative importance of environmental and spatial control and to compare the…
Metacommunity dynamics of Ostracoda in temporary lakes: Overall strong niche effects except at the onset of the flooding period
2017
Abstract Metacommunity research usually focuses on the structure of species assemblages and their influencing factors, chiefly environment and space. However, the temporal dynamics of metacommunities and their structuring processes are rarely investigated. Here, we analyze the temporal variations in a metacommunity of ostracods from temporary shallow lakes of the Iberian Peninsula. Our aims were to determine the variability of the ostracod assemblages throughout a hydrological cycle by means of partial triadic analysis (PTA), and to analyze the response of these communities to both environmental and spatial variables. The metacommunity was moderately stable through the study period, with la…
Spatial and environmental effects on a rock-pool metacommunity depend on landscape setting and dispersal mode
2017
Empirical studies on structuring mechanisms of metacommunities usually focus on the major roles of environmental filtering and dispersal. Recent works suggest that the relative importance of these structuring mechanisms differs among organisms with different body size, taxonomic affiliation, and dispersal abilities, and also depends on spatial extent and environmental heterogeneity. However, the effects of physical connectivity among sites and dispersal mode are less commonly considered explicitly in field metacommunity studies. We analysed a rock‐pool animal metacommunity, comparing both environmental and spatial effects between a set of pools in a ravine setting, with ephemeral connecting…
Active packaging with antifungal activities.
2016
International audience; There have been many reviews concerned with antimicrobial food packaging, and with the use of antifungal compounds, but none provided an exhaustive picture of the applications of active packaging to control fungal spoilage. Very recently, many studies have been done in these fields, therefore it is timely to review this topic. This article examines the effects of essential oils, preservatives, natural products, chemical fungicides, nanopartides coated to different films, and chitosan in vitro on the growth of moulds, but also in vivo on the mould free shelf-life of bread, cheese, and fresh fruits and vegetables. A short section is also dedicated to yeasts. All the ap…
Distribution of sea urchins living near shallow water CO2 vents is dependent upon species acid-base and ion-regulatory abilities.
2013
To reduce the negative effect of climate change on Biodiversity, the use of geological CO2 sequestration has been proposed; however leakage from underwater storages may represent a risk to marine life. As extracellular homeostasis is important in determining species' ability to cope with elevated CO2, we investigated the acid-base and ion regulatory responses, as well as the density, of sea urchins living around CO2 vents at Vulcano, Italy. We conducted in situ transplantation and field-based laboratory exposures to different pCO2/pH regimes. Our results confirm that sea urchins have some ability to regulate their extracellular fluid under elevated pCO2. Furthermore, we show that even in cl…
Modelling the effects of more selective trawl nets on the productivity of European hake (Merluccius merluccius) and deep-water rose shrimp (Parapenae…
2018
Single-species Gadget models were used to assess the effects of using a sorting grid mounted on the traditional trawl net used by Sicilian trawlers to exploit the deep-water rose shrimp in the Strait of Sicily. The main commercial by-catch species of this fleet is the European hake (Merluccius merluccius), often caught at sizes well below the minimum conservation reference size. Selectivity curves based on the results of an experimental survey carried out in the area using a commercial trawler equipped with an ad hoc-designed sorting grid were incorporated into single-species Gadget models to forecast the effects of changing fishery selectivity on the performance of the two stocks in terms …